FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005334023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005334023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178758
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA802.765435E-630.18564846
GTCGCCG902.1289154E-422.99814644
TCGCCGT1550.00811882813.35402145
GCCGTAT2551.1245512E-412.17572447
TAGATCG5907.018604E-57.60078868
CGTCGTG5000.006488346.89917768
AGCGTCG5700.00310395246.657164669
GCGTCGT5200.0094750176.63395267
GTCGTGT5950.00486307826.377390468
ATCGGAA13455.97307E-75.6424568
TCGGAAG12808.544508E-65.39003469
AGATCGG13604.3496566E-65.32672566
GATCGGA13101.27987205E-55.266649267
Produced by FastQC (version 0.11.5)