FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005334048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005334048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5062581
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1903.500263E-718.1594146
CTCGTAT2554.6037894E-714.8813942
TCGTATG2801.3224217E-613.55336443
CGTATGC2952.3768007E-612.86421144
TCTCGTA2601.3474516E-411.9418941
ATGCCGT3451.644103E-410.00083447
TGCCGTC3552.1891855E-49.72065848
GCCGTTT3600.00268608878.62486859
ACACGTC4200.00117628338.21359213
GCCGTCT3800.00436756578.17302649
CGTCTTC5007.7269087E-47.591833651
GATCGGA5500.00209788426.9097911
TCGGAAG5750.00339958196.59963753
ATCGGAA6050.0057806846.27238372
CACACGT7954.0028218E-46.07495912
CCGTCTT6300.00876886.025264750
Produced by FastQC (version 0.11.5)