FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005334049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005334049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5062581
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1401.3918907E-824.64009946
GTCGCCG1851.1801603E-820.51121544
GCCGTAT1954.6056448E-717.69050247
TCGCCGT2101.0002859E-616.42673145
CGTCGTG2708.779916E-714.05509312
GCGTCGT2609.175528E-613.26879411
CGTATCA2801.9691528E-512.3194449
CCGTATC3154.956295E-612.04638948
AGCGTCG2902.8181243E-511.895925510
GGTCGCC3257.020655E-611.67596243
TGGTCGC3904.5486668E-610.61451142
TCGTGTA3602.1814087E-510.54131914
CGTGTAG4108.25445E-610.09732815
CTCGGTG4452.1778187E-59.30407537
GTCGTGT4452.21484E-48.52780913
TCTCGGT4400.00185913927.84127136
TCGGTGG5805.664723E-57.732962638
AGAGCGT5653.527599E-47.3269758
CGGTGGT6603.5263125E-57.318374639
CCGGGGT6250.00111758076.62293149
Produced by FastQC (version 0.11.5)