FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005334336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005334336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4319987
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5003.5612175E-713.1109291
ATCGGAA7252.9346447E-69.7786832
TCGGAAG7001.1245003E-58.7972353
ACACGTC3904.7724734E-68.50166712-13
TCTCGTA3459.088178E-58.23783140-41
CGGAAGA8702.340057E-68.1672884
CTCGTAT3802.7854514E-47.47908442-43
CGGCAAT3903.7559902E-47.28722834-35
ACTCGGC5001.6829354E-57.105211732-33
CGTCTGA4951.1290614E-46.698283716-17
AGATCGG5701.4584632E-56.648074694-95
TCACTCG4300.00114370886.60957630-31
TCGGCAA5150.00131765285.97835734-35
AATCTCG6100.00165087125.435586538-39
CACTCGG6000.0080277125.131601330-31
ATGCCGT6600.0044224715.024061746-47
Produced by FastQC (version 0.11.5)