FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005334404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005334404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5518527
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2350.022.0205946
CTCGTAT3605.802576E-1014.37468342
CGTATGC3454.9130904E-913.99956444
TCTCGTA3406.1207174E-813.19004941
ATGCCGT3601.2901364E-712.45794747
TCGTATG3902.735942E-812.38434343
TGCCGTC3903.6490565E-711.49974748
CGTAGAG4055.945185E-711.073729533
GTCACGT5553.274181E-89.94545629
GCCGTCT4653.50142E-69.64451149
AGTCACG5705.1194183E-79.078528
TCACGTA6101.3590379E-68.48341930
CGTCTTC5704.9922637E-68.47311351
ACGTAGA5857.0748756E-68.25615332
CCGTCTT7001.1416269E-67.885182450
CACGTAG6705.1825446E-67.723710531
TCGCACC4550.00258728357.58183861
TAGATCG6958.695777E-67.446351567
ATCTCGT5804.7661472E-47.13732140
GATCGGA19250.06.63174469
Produced by FastQC (version 0.11.5)