FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005334406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005334406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6419093
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAT1359.447831E-925.55484835
TATGCCG2304.27822E-513.49941646
ACGCCGC2752.2701395E-411.29059632
CACGCCG2802.683E-411.0889831
CGTATGC3301.0504994E-410.45401344
CTCGTAT3702.952253E-510.25615442
TCGTATG3803.9590443E-59.98625643
GCCGCGA3652.9023737E-49.45179334
ATGCCGT3804.3454906E-49.07855447
TCTCGTA3905.635719E-48.84522141
CGCCGCG4058.198522E-48.51828433
AGTCACG5650.00282828426.716471728
CGCGATC5150.0086276896.698791536
CAGATCG7900.00243980625.67763368
ATCGGAA18251.886292E-95.48259569
GATCGGA19055.467882E-95.25235568
GCGATCT9950.00120615525.200496737
Produced by FastQC (version 0.11.5)