FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005334426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005334426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5579124
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3103.6379788E-1217.80671546
CTCGTAT4151.8189894E-1214.96340742
TCTCGTA4001.2732926E-1114.66206141
CGTATGC4301.8189894E-1214.44142744
TCGTATG4152.3646862E-1114.13210743
TGCCGTC4551.07320375E-1012.89030148
ATGCCGT4803.4033292E-911.49955247
GCCGTCT5057.530616E-910.93085549
CGTCTTC5959.577161E-89.27736651
ATCTCGT6655.246493E-78.30057740
TGACGCA4150.0096500357.48123415
CCGTCTT8653.674293E-66.78044750
TAGATCG8754.3814E-66.70451969
GTATGCC9452.0760926E-66.571525645
ACACGTC10058.2102997E-76.52201713
CGTCTGA13053.3269862E-75.81576116
GATCGGA22451.4551915E-115.379952469
AATCTCG11051.5700347E-45.307580539
GCACACG15804.327303E-75.241805669
AGATCGG22651.09139364E-105.18009268
Produced by FastQC (version 0.11.5)