FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005343007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365616142
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG47420450.34724582217189404TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA12280050.068.558711
TCGGAAG12653100.066.387462
CGGAAGA13152500.063.8258173
AGAGCAC20687650.040.9257627
AGCACAC21459400.039.4047789
GAGCACA21953600.038.5655068
GAAGAGC22457850.037.7466855
AAGAGCA30104300.028.4135746
GGAAGAG33221400.025.8253844
CGCGCAT7636750.022.03237535-39
TATGCCG7860000.021.98664345-49
TCGCGCA7906600.021.57618935-39
TCTCGCG8128800.021.27656430-34
GCGCATC8145100.020.76818835-39
CGTATGC8300750.020.62549645-49
CTCGTAT7711300.020.59764140-44
TCTCGTA7782200.020.13593540-44
ATGCCGT8633550.019.84857645-49
GCCGTCT8647750.019.47331850-54
CTCGCGC8617700.019.44871530-34
Produced by FastQC (version 0.11.5)