FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837917
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7500.049.918368
CTGTCGC9850.038.0089049
GTCGAAA850.002694539334.2408261
GTCTCGC8300.032.4359631
GTCTTGC20150.031.4157431
TCGCTCT8300.030.360454
GTGTTAC8450.030.1380021
GTATTAA6150.029.577951
GTATAAT6100.027.434761
TCTCGCT9500.026.5304952
GTAGTAC3555.456968E-1226.3717883
GTCTCAC22650.024.4145411
GTCTAAC5150.024.0184441
CTCGCTC9300.024.0050893
TTGCTCT25550.023.9522444
TAACACT9700.023.7517994
GTGTAGG10150.023.656531
GGGTTAC5550.023.5984061
GGATAGT5600.023.3877051
GTGTAAC5600.023.3877051
Produced by FastQC (version 0.11.5)