FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837917
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.040.215628
GTCTCGC9250.035.8992161
GTCTTGC19050.034.4753951
CTGTCGC11400.030.9413349
GTCTTAC6500.030.6524091
TCGCTCT9500.030.3090534
GTATTAT5500.029.5171361
GTATTAC4650.028.564971
TTATACT6750.027.7271734
GGTATAC4000.027.6723141
CGCTCTG10550.027.2925115
CTCGCTC10600.027.1637763
GTATAAA7350.026.1035881
TCTCGCT12950.025.0212352
GTGTAAA12150.024.9012871
GTGTTAG12500.024.7943941
GTATTAG6300.024.5976121
GTGTTAT7350.024.095621
ACCGTGT6300.023.995328
TAATACT6000.023.9946694
Produced by FastQC (version 0.11.5)