FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139712
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.040.6933948
CTGTCGC15600.030.921369
GTCTCGC15150.030.2501411
GTCTTGC29200.029.396711
GTGTAAC9300.028.9413171
TCTCGCT16400.026.7819862
GCGTTAT1958.536326E-626.1133751
GTACTAC5300.026.0781781
TTCACCG8950.025.7411775
TCGCTCT16350.025.0991234
GGGTACG3501.2369128E-1024.9409331
GGTATAC7000.024.9409331
GTCTCAC35950.024.88891
CTCGCTC17500.024.6874893
GTTTCAC50550.024.642723
GTATTAG9950.024.1262821
GGGTTTC57800.024.038371
GTCTTAC12350.023.5609361
TTGCTCT38250.023.527734
GTATTAC7200.023.2377911
Produced by FastQC (version 0.11.5)