FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139712
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13400.042.4011961
TCTGTCG14300.038.2572948
TCGCTCT15050.036.3507844
GTCTTGC28300.032.070881
CTCGCTC16550.031.7513033
CGCTCTG17650.030.5881525
GTCGTAC1702.627503E-630.3837451
CTGTCGC20100.028.6503939
TCTCGCT18350.028.2534482
GTATTAG11500.027.5907061
GTGTTAG22650.026.7139321
GTATAGG7200.024.5963651
GTATAGA9450.023.4251081
GGTATAC6000.023.3665471
CCGTGTT11100.023.3461999
GTGTAGG17400.022.4759861
GTCTCAC35850.022.4351771
TTACACT15100.022.4056514
GTGTTAC14250.022.2661821
TTCACCG10700.022.200715
Produced by FastQC (version 0.11.5)