FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227954
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14150.045.79358
GTCTCGC16350.038.272831
CTGTCGC18850.036.285249
TCGCTCT17050.035.048124
TCTCGCT19150.030.0819682
CTCGCTC19050.029.485253
CGCTCTG20050.028.7267575
GTCTTGC30150.028.4776781
GTCTTAC13050.026.7633611
GTATAAG9700.024.7543831
GTTTCAC50400.024.4326613
GTATAAC6300.024.2542931
GTATTAG10250.024.135981
GTGTAAT15100.024.093671
GTCTCAC38500.024.0023021
TTCACCG8650.023.3052065
GGGTTTC56000.022.8683361
GTATTAA9250.022.8121451
TTATACT9600.022.4988864
TTGCTCT42450.022.3875854
Produced by FastQC (version 0.11.5)