FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227954
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16400.044.096361
TCGCTCT15950.041.5201574
TCTGTCG14550.038.5895968
GTCTTGC29550.034.4621281
TCTCGCT20250.034.1375852
CTCGCTC18900.033.135183
CGCTCTG19050.031.7406775
GTATTAG10650.030.4876211
CTGTCGC20800.026.9941629
GTTTCAC51450.025.7433053
GTACACG4101.8189894E-1225.197921
GTGTTAG23500.024.1792771
GGAGTGC72400.024.1601475
TTGCTCT41950.024.0230374
GGGTTTC55650.023.7360461
GTATTAA9700.023.5836181
TAATACT11300.023.569764
TACACTG19800.023.2673365
GTACTAG6350.023.2421721
GAGTGCA80550.023.056116
Produced by FastQC (version 0.11.5)