FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3210878
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.042.8267568
GTCTCGC12850.035.10051
GTCTTGC28600.034.848111
CTGTCGC16700.029.31689
GTCTTAC11450.027.320441
TCGCTCT14650.027.0301954
GTCTCAC35350.025.7244031
TCTCGCT16550.024.8017392
GTGTAAC10000.024.734531
GTGTTAC12900.024.2495381
GTATAAT9600.024.2495381
CTGTTGC31550.023.9615029
CGCTCTG15750.023.770975
CTCGCTC16100.023.7076823
GTATTAG8500.023.1083851
GTATTAT10750.023.0088631
GTTTCAC47750.022.9253
GGGTTTC51000.022.8230951
GTGTAAT12150.022.7526551
GTATAAC6800.022.4664841
Produced by FastQC (version 0.11.5)