FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309485
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13550.041.3650551
TCTGTCG12150.039.7024238
TCGCTCT14600.036.490874
CTGTCGC16450.033.2633639
GTATTAG11000.031.7636741
TCTCGCT17300.031.221362
CGCTCTG15650.030.3623225
GTCTTGC29550.029.8064381
CTCGCTC16900.029.404393
GTCGTAC1255.0365773E-429.1167011
GTACTAG5650.027.0553421
TTACACT15050.024.8755234
GTACCGT1801.4804269E-424.2639181
GTGTTAG20350.023.9658361
GTGTTAC13100.023.8934761
GTATTAC9000.023.455121
TTCACCG9850.023.3894025
GTATAAT12450.022.8022351
GTGTAAA19500.022.7707561
GTATAGG8150.022.328761
Produced by FastQC (version 0.11.5)