FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309485
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13600.037.493591
GTCTTGC28450.034.027961
TCTGTCG13750.032.457968
TCGCTCT15450.030.750184
TCTCGCT16950.028.036552
CTCGCTC17350.027.3827253
TTGCTCT38700.027.3425224
TTCACCG8450.025.5562275
GTATTAG10800.025.3177341
ACCGTGT9250.024.1241598
CCGTGTT9650.023.8701349
CGCTCTG16650.023.778295
TCTGTCA39500.023.6907318
CTGTCGC19450.023.3159529
GTCTTAC12100.023.2083951
GTCTCAC35100.023.1596741
CTTGCTC37300.023.158193
GGTATAC7750.022.885291
GGGTTTC51700.022.2987691
TCACCGT10700.022.200486
Produced by FastQC (version 0.11.5)