FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067150
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12400.038.9053968
GTCTTGC28350.036.715771
GTCTCGC12100.033.0862051
CTGTCGC15350.030.959389
GTATTGG9500.026.8172421
GTATTAG8700.026.7732071
TTGCTCT41100.026.6283534
TCTCGCT14700.026.4526082
CTTGCTC39400.025.7729743
GTGTTAC12500.025.6219581
TCGCTCT13800.025.5658074
GTGTAAC10350.025.3181421
GTGTACG4051.8189894E-1225.1618561
GTCTTAC11500.024.6851861
GGTATAC6600.024.2632181
TCTTGCT44850.023.7624592
GTGTTAG20950.023.6262341
GTATAAT8700.023.4265561
GTATAAC6250.023.2926881
CTCGCTC16200.023.1169223
Produced by FastQC (version 0.11.5)