FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905233
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.036.632458
TCGCTCT12850.036.4198464
GTCTTGC27100.034.6556471
GTCTCGC13250.034.066651
CTGTCGC14050.031.2605299
GTATTAA9300.029.7477591
TCTCGCT15650.029.446312
CTCGCTC15850.027.7142543
TCTCGAT9650.027.6068737
CTCGATC9800.027.184328
CGCTCTG15950.027.0843495
TCGATCT10550.026.6167459
GTCTCAC35600.025.7676091
GTATTAG10300.025.4459611
TTACACT15450.025.1647824
GTCTTAC11550.024.5830751
TTGCTCT38350.024.2187774
GTGTAGG16900.024.124311
GTATCGC1550.001765835323.4850711
GTATAGA9650.023.3877221
Produced by FastQC (version 0.11.5)