FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881357
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13700.035.5873571
TCTGTCG10350.034.7809648
GTATTAG10600.032.951631
TCGCTCT15300.032.939624
GCGTTAG2003.1543095E-729.1072711
GTACTAG5750.029.1072711
CTCGCTC16750.028.8061393
TCTCGCT17550.027.0812842
CTGTCGC15050.026.3110499
GTATAAT8650.026.078771
CGCTCTG17800.025.0774695
GTATAAG9000.025.0645941
GTCTTGC28600.024.9345511
TACCCGT2651.4742363E-724.4516755
TATACTG9550.024.1245165
GTATTGC7350.023.7610361
GGGTACG2502.2096265E-623.2858161
TTACACT16100.023.253564
TTTACGC1550.001881867823.2295482
GTATTAC7850.023.174581
Produced by FastQC (version 0.11.5)