FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881357
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC25550.030.0341821
GTACCGA1453.6665904E-529.7871256
GTATTAG10550.029.374481
TCTGTCG14150.027.9797658
GTGTTAG20450.027.7824651
GTCTCGC13700.026.3905751
GTATTAT8550.025.8897881
GTCTTAC11700.025.8565961
TTCACCG8650.024.9660875
GTTAGCC14850.024.722313
CCGTGTT8450.023.8527859
GTGTTAC12950.023.3607851
TGTTAGC18000.023.1993712
TACCCCG3153.0948286E-822.8525565
GGTATAC5900.022.5109521
ACCGTGT9800.022.0360138
GTATTAC7750.021.8977491
GGATAGG12900.021.7353781
TTGCTCT37900.021.6526454
GTCTCAC34850.021.5958731
Produced by FastQC (version 0.11.5)