FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829560
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.042.942128
GTCTCGC12250.039.842971
TCGCTCT13000.038.2107124
CTGTCGC13250.038.033099
CTCGCTC14050.032.8012583
GTCTTGC24450.032.1779171
CGCTCTG14300.031.2129695
TCTCGCT14650.028.5091932
GTATTAA9050.028.1729621
GTCTCAC31450.026.8689271
GGGTTAT7650.026.6630341
GTACTAT5500.026.48991
GTGTAAA15800.026.2803291
GTATTAT9400.024.7990571
GTACTAC4750.024.5380131
GTGTTAC9950.024.1603871
TCACTCT34600.023.9277114
GTATTAG8850.023.8708441
GTGTAGT13400.022.8327121
GTGTTAG15400.022.705631
Produced by FastQC (version 0.11.5)