FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829560
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13200.046.3526428
GTCTCGC13400.044.6717031
CTCGCTC13900.039.8782463
TCGCTCT14000.039.5927054
TCTCGCT15850.036.8324362
CTGTCGC16300.035.7706539
CGCTCTG15050.033.9605185
GTGTAAA14850.031.8497311
GTCTTGC23850.031.2957441
GTACCGA1100.00881238626.873211
GTCTTAC9500.026.4488981
GTGTTAG18700.025.2924371
TACACCG2851.0690201E-825.2585035
GTATTAG8650.024.776171
TACACTG15750.024.224115
GTGTTAC10750.024.06091
TAATACT8450.023.8535944
GTTTCAC40850.023.7904073
GTATTGG6600.023.5140611
TTGCTCT35250.023.4850334
Produced by FastQC (version 0.11.5)