FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837808
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12850.039.1069681
TCTGTCG12300.034.5257958
TCGCTCT13900.033.1413044
GTCTTGC26000.030.6971991
TCTCGCT17800.028.7465932
GTCTTAC11350.027.997611
CTCGCTC16300.027.8239383
GTATTAC6800.026.0825881
CGCTCTG16750.025.7834385
GTGTTAC11800.025.677351
GTCTCAC32100.025.3242171
CTGTCGC16400.025.016579
GTACTAG5750.024.4193521
TTGCTCT35550.023.4867444
TAGTACT6200.023.2189564
TCTGTCA36600.022.8125428
GTATAGG6850.022.6556781
GTGTTAG18400.022.4915071
GTACTAT6000.022.17021
GTATTAG7900.021.5153851
Produced by FastQC (version 0.11.5)