FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477493
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11050.042.348318
TCGCTCT11550.039.8925484
CTGTCGC12000.038.9957359
GTCTCGC12000.038.870411
GTCTTGC24350.035.617911
TCTCGCT14350.034.118872
CGCTCTG13950.031.4810645
CTCGCTC14550.031.1756233
GGTATAC4400.026.5025561
TTGCTCT34100.026.390614
GTGTAAC8000.025.508711
GTATTAA7600.024.9333251
GTATAAT7150.024.4638961
GTGTTAG17050.024.3652531
GGGTTTC44850.023.8877541
GTTTCAC42450.023.067413
GTGTAGT15900.022.9188771
GTCTCAC31300.022.5864431
CCGTTCG1600.00226678322.497549
GTGTACG3253.7794962E-822.425241
Produced by FastQC (version 0.11.5)