FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669477
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9350.038.4967738
CTGTCGC12950.028.9067679
GTCTTGC21050.028.7743241
TCGCTCT11050.028.0138074
GTACTAG4450.026.2385061
GTACCGA1409.6244115E-426.0628011
GTCTCGC10400.025.962561
CTCGCTC11450.025.7835043
GTACCGG2001.0135731E-525.5415461
GTGTAAA14200.024.1539781
TAGCCCG1801.5959796E-423.996325
CGCTCTG12150.023.7000715
GTGTAGG11400.023.0450061
CTTGTCG2502.4262354E-623.036479
GTGTAAT8700.022.6476761
GTCTCAC26400.022.3903161
TAGTACT5150.022.3655034
TAGACCG1650.002711057721.8148385
GTATTAT8700.021.8088741
TTGCTCT30700.021.5732424
Produced by FastQC (version 0.11.5)