FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669477
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.036.49778
GTCTTGC20500.033.586781
GTCTCGC10800.032.9046131
TCGCTCT11250.027.5145034
GTATTAG6950.026.6314331
CTGTCGC15600.026.3009599
GTATTAA7350.026.1893881
GGGTACG2309.1985567E-725.7514381
GTGTTAG13600.025.0413781
CTCGCTC11950.023.4932173
GTGTAAG10450.023.3795931
TACTCCG1851.9223354E-423.3466875
TCTCGCT14000.023.166012
CTTGCTC27200.021.9662023
CGCTCTG13400.021.4882455
GTATTAT7100.020.8550381
GGCTAGA13500.020.8395881
GTCTCAC26850.020.6802751
GTCTAGA10400.020.6444821
TCTGTCA27900.020.639888
Produced by FastQC (version 0.11.5)