FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812867
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13150.041.606568
CTGTCGC15700.034.390279
GTCTCGC12550.031.957981
TCGCTCT14000.030.3398484
GGGTACG2802.4556357E-1028.6480431
TCTCGCT14400.027.503632
GTCTTGC27000.027.278341
CTCGCTC15050.026.7942353
GTGTTAC11500.025.3642771
GTCTTAC11350.025.0571
GTGTAGG14200.024.6497921
GTGTAAT12850.024.4020161
GTATTAG10200.024.3074321
CGCTCTG16400.024.1439485
TACGACA1500.001557314223.9976224
GTGTTAG19450.023.9949971
GCGTAGT3102.277011E-823.5233231
GTGTATA15250.023.430771
TACGCTA1550.001884706423.2235055
CCGTGCG3102.6100679E-823.222689
Produced by FastQC (version 0.11.5)