FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385077
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG6800.032.060941
GTCTCGC10400.031.4443821
TCTGTCG9200.031.3008678
TCGCTCT10900.029.7214624
GTGTTAG11650.028.6943111
GTCTTGC18350.026.9300231
CGCTCTG11950.026.5075135
TCTCGCT11650.025.3388942
GGAGTGC48950.024.4140385
GTACTAC3901.8189894E-1124.2238221
GTATAAC3952.1827873E-1123.917191
CTCGCTC12350.023.9026813
CTGTCGC12550.023.5192959
GAGTGCA55400.022.7411546
CTGGAGT57900.022.507633
GTCTTAC7750.022.5047131
GTGTAGG10450.022.2534621
GTATAAT5300.021.9385551
GTCTCGA20450.021.8264226
TCTGTCA23300.021.6284948
Produced by FastQC (version 0.11.5)