FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385077
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT750.001536586438.3925554
TCTGTCG9600.033.7420438
GTCTCGC10550.030.7306861
GTATTAA5100.030.3403911
GTCTTAC6900.029.9006771
GTACTAG3750.029.4735241
GTCTTGC19400.027.726381
CTGTCGC12950.026.6809649
GTCTCAC21550.025.9859371
GTATAGC4000.025.7893331
TCTCGCT12550.025.267272
CTCGCTC11750.025.1195873
TCGCTCT10950.024.9814554
GTATTGG5650.024.7786251
GTATAAG6400.024.17751
GTGTTAT6800.023.8388791
GAGTGCA51650.023.8322436
GTTAGCC11200.023.7820993
GTACTAC2802.0059451E-723.6840781
GGAGTGC49200.023.263785
Produced by FastQC (version 0.11.5)