FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562332
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.043.316688
GTCTCGC13750.041.3256651
TCGCTCT13200.037.631714
CTGTCGC13900.037.290359
CCGATAC800.002105035136.0089043
GTCTTGC25500.035.9963381
TCTCGCT16000.032.408012
GTACTAT5950.031.8334961
GTATAAC4150.031.5974731
CTCGCTC15550.031.030183
CGCTCTG15600.029.5348115
TCGATCT9700.026.7183489
CTCGATC9750.025.8429588
GTGTTAG18050.025.4267751
GGGTTTC44700.024.1202681
TCTCGAT10150.024.1152467
TTGCTCT34350.024.1018264
TTCACCG6700.023.638875
TACACTG16450.023.6323385
TAATACT8300.023.4187934
Produced by FastQC (version 0.11.5)