FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562332
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.046.5407188
GTCTCGC11350.041.6894531
CTGTCGC16050.036.3278589
GTCTTGC25850.035.4652671
TCGCTCT12900.034.038464
GTATTAA8100.031.0338751
CGCTCTG14700.028.4014455
GGTATAC4950.028.3785721
TCTCGCT14250.028.3261892
CTCGCTC14000.028.2806823
TTGCTCT34850.027.6778134
GTACTAT5750.027.0018541
GTATAAT7500.026.6161121
GTATTAG8200.025.2456361
GTATTAC6200.025.042041
GTGTTAG18550.024.7109761
GGATAGT6300.024.6445481
GCGTAAC1500.001333750824.6445481
GTCTCAC30200.023.5020851
GTGTAAT11650.023.4810711
Produced by FastQC (version 0.11.5)