FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118573
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG7300.030.4043561
TCGCTCT13450.029.435794
GTCTCGC12900.026.9553871
GTCTTGC23650.025.6519341
TCTGTCG12650.025.0370718
CTCGCTC14200.022.8125293
GTATCCG1952.2811707E-422.7642861
GTCCGGA3603.8435246E-922.6062031
GGAGTGC60250.022.2224125
GTCCTAG11800.021.9443871
CTAACGT2303.322227E-521.9088423
ACTCCGT8550.021.8891338
GTATTAA7100.021.8825721
GGTATAA5500.021.52261
GTGTTAA8600.021.5069581
GTGTTAG16900.021.4509621
GTCCTAC7250.021.4298291
CGCTCTG14850.021.328565
GAGTGCA65100.021.0087856
GTACTAA5351.8189894E-1220.7431581
Produced by FastQC (version 0.11.5)