FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318683
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGGCCATATCTCGTATGCCGT34630.14935202440350837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8750.037.8468368
GTCTCGC10050.034.044171
TCGCTCT11150.033.57444
CGTCAGA3400.032.1161351
GTACGAG2003.1431045E-729.1186311
CTGTCGC11550.028.6718469
TCTCGCT11650.028.4330922
CTCGCTC12200.028.3317643
GTCTCAC23850.028.0808621
GTATTAG5550.026.2329981
CGCTCTG11750.025.1203585
GTACACG2401.543749E-624.2655261
GTGTAAC6100.023.8677311
GTCTTGC20500.023.7920531
GTCTTAC8500.023.1236171
CGCTCTA1600.002266897122.497275
GTATAAG6300.021.9545231
GGCTAGA13500.021.030121
CCGCATG3851.14196155E-820.5689329
GTGTTAC7450.020.5198421
Produced by FastQC (version 0.11.5)