FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681965
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11850.039.889981
TCTGTCG12100.039.269698
CTGTCGC14550.034.6371429
TCGCTCT14050.031.7739334
CGCTCTG15250.031.1576635
TCTCGCT15050.030.1608242
GTCTTGC25550.029.601331
CTTATCG1255.3710747E-428.7983119
GTATTAG7600.028.706251
GTGTTAG18900.028.0885851
GGGTAAG13450.026.4936981
GTGTTAC12300.026.0145511
CTCGCTC15850.025.4564513
GGGTTTC47350.024.2664321
GTCTTAC10200.024.2408331
GTTTCAC45050.023.9902953
TTGCTCT35400.023.7978784
TAACACT12750.023.1541734
GTATAGC6600.023.1389771
GTGTAAG12900.023.1133521
Produced by FastQC (version 0.11.5)