FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357484
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTCAATATCTCGTATGCCGT35110.14892996092444316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10300.038.141951
TCTGTCG9650.036.5560658
TCGCTCT12800.033.7531934
GCGTAGA2151.7524144E-830.4544261
CGCTCTG13700.029.953325
GTCTTGC21650.029.9073851
CTCGCTC13550.029.7750633
CTGTCGC12600.029.1412859
TCTCGCT13200.028.9271662
GTACTAG3301.8189894E-1228.6599731
CGTCAGA4500.025.867461
GTATACG2101.5149268E-524.2507441
GTACTAT3352.0299922E-923.8887941
GTGTTAG12200.023.8531931
GTATAGA5800.023.8326261
GTACGGG1600.0021325322.7350731
GGTCTCG22550.022.667445
GTATAGC4204.9112714E-1122.5185491
GTCTCGA23100.022.1277376
GTGTAAG9000.021.825671
Produced by FastQC (version 0.11.5)