FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816171
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.046.408618
CTGTCGC16050.036.333489
GTCTCGC13600.031.5390511
GTCTTGC27250.030.4140241
GTATTAG8050.029.802561
TCGCTCT13800.029.7398724
CGCTCTG15500.026.9397355
TCTCGCT15950.026.1968922
GCGTATA1409.839487E-425.9643541
GTCTCAC32000.025.2178781
GTATAAT7800.025.165451
CTCGCTC16100.025.0578983
TACACTA5750.025.0414335
GTATAAA10600.024.690631
GTGTAGA13700.024.4102821
CTCGATC11550.024.3092738
TTGCTCT36300.024.198954
GTGTAGG15700.023.1529251
GTGTAGT14650.022.8273621
ACCGTGT8900.022.6494358
Produced by FastQC (version 0.11.5)