FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816171
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12950.035.9252971
TCGCTCT14400.035.4806444
GTCTTGC27900.034.131651
TCTGTCG12750.033.2994848
CGCTCTG15250.031.1436675
GGTATAC5150.029.641641
CTCGCTC16350.028.6082483
CTGTCGC17100.026.0910349
TCTCGCT18300.025.9530582
CGTTTCG1959.284526E-625.8320629
CTCGATC12300.025.7420588
GTGTAAA15750.025.3847141
TTGCTCT36600.025.3632164
TATACTG8500.024.5513155
GTATAAG8100.024.2308651
TATTCCG2401.7071852E-623.9869175
GTATTAA8500.023.9457971
GTCTTAC10650.023.8895851
TCGATCT12950.023.338629
GTATATA11000.023.1294611
Produced by FastQC (version 0.11.5)