FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855792
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12800.043.870988
CTGTCGC15350.037.9906739
GTCTCGC14250.033.1695441
GTCGTAC1102.394717E-433.053571
TCGCTCT17100.032.421494
GGTATAC5150.031.0639341
GTCTTGC27300.029.5665971
CTCGCTC17500.027.5805743
TCTCGCT16850.027.3619232
GTACTAC5100.025.6651251
CGCTCTG18150.025.3861055
GTATAAT7700.024.5540771
GTGTAGT16000.024.5422761
CTCGATC12050.023.8982288
GTGTTAC13850.023.626741
GTCTCAC34550.023.572791
GTGTAAT10550.023.4351371
GTGTTAG20550.023.3546371
TACTACG1851.9209729E-423.349217
TCGATCT12250.022.920759
Produced by FastQC (version 0.11.5)