FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855792
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13550.039.152241
TCTGTCG13350.037.7313888
GTCTTGC27100.030.5709271
TCGCTCT15250.029.7279014
CTGTCGC18150.029.3387139
GTATTAA8050.028.8886451
GTTACGC1255.386078E-428.7846643
GTATTAT9700.027.7206271
TCTCGCT17050.027.4340632
GTGTAAG13300.026.2278481
CTCGCTC16900.025.9743273
CGCTCTG16700.025.8540385
GTCTTAC10800.024.8972321
CTAGTAC4400.024.5323853
GTATATA12000.023.6187231
GTCTAGT11900.023.2065011
TCGAACT16500.023.114159
GTACAGG16150.022.9493661
TTGCTCT37100.022.8876744
CTGTTGC28350.021.0674049
Produced by FastQC (version 0.11.5)