FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005343997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005343997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973035
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.042.9920588
GTCTTGC26600.036.3617971
GTCTCGC12200.035.7657051
CTGTCGC14650.033.9087339
GTATTAA9600.030.3014971
GGTATAC6050.030.0510731
TCGCTCT13900.029.5274494
TCTCGCT15550.026.413862
TTGCTCT38600.025.3698274
CTCGCTC16800.024.8774643
GTATTAT9050.023.3036941
GTGTAAT10650.023.2169231
GTGTATA16050.023.108431
CTTGCTC37850.022.8456123
GTGTTAG19750.022.8296871
TTAACGC1600.002254716122.5183963
CGCTCTG16850.022.2175565
GTGTAAG14600.021.91671
GTCCTAC8350.021.7735311
GTGTAGA16100.021.6815681
Produced by FastQC (version 0.11.5)