FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853619
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11450.037.0973558
GTCTCGC14350.035.4551241
GTATACG2008.745701E-932.7073481
GGTATAC5600.032.4477651
TCGCTCT15250.032.106194
CTGTCGC13700.031.0047239
GTATTAG10350.030.19681
TCTCGCT16400.029.4359132
GTATTAT8650.026.8885081
CTCGCTC17150.026.8882453
GTCTTGC25400.025.4676651
GTACTAG5750.025.2810441
GTATTAA9150.024.6248421
CGCTCTG18400.023.867585
GTCTTAC10900.023.3385771
GTCTAAT8850.022.9957521
GTGTTAG19800.022.3922371
CTATACT9600.021.750864
GTGTAAT11800.021.5585161
GTATAAG8100.021.5357021
Produced by FastQC (version 0.11.5)