FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853619
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13050.032.8277931
TCTGTCG13350.031.2631788
GTCTTGC26550.030.6304741
CTGTCGC16650.027.227819
TCGCTCT12950.027.2268544
GTATAGA8450.024.9196241
GCGTTAC2051.2761813E-524.7938751
GTGTTAG19250.023.386281
GTATTAG10900.023.315341
TCTCGCT15900.023.0804462
GTGTTAC12150.022.7094971
GGTATAC5450.022.6491871
GTGTAAT12300.021.8422221
GTATTAT9100.021.5438141
CTCGCTC15700.021.0828513
GTATTAC6700.020.5910761
CGCTCTG16850.020.4984055
TTGCTCT38700.020.4528054
GGCTAGA18650.020.2453231
GTGTAAG14550.019.9616851
Produced by FastQC (version 0.11.5)