FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817063
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.039.7618838
GTCTCGC13000.034.6792531
TCGCTCT12750.034.4468154
CTGTCGC14800.032.5920529
GTCTTGC28450.030.926051
CGCTCTG15250.028.7972415
TCTCGCT14550.027.7293572
CTCGCTC15500.026.0298163
GTATTAG10950.025.8983361
GTATTAA9250.024.3692021
GCGTAAT2202.1802687E-523.1364521
GTGTAAC9650.022.6055621
GTATAAT8800.022.310151
GTATATA12150.022.1435281
GTATTGG10200.022.0995221
GGAGTGC65350.022.0330855
GAGTGCA72900.021.825076
GTCTTAC10500.021.4681071
TTATACT8100.021.3331814
GGGTTTC53500.021.202751
Produced by FastQC (version 0.11.5)