FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208007
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14350.040.135678
GTCTCGC14400.039.854151
TCGCTCT16600.036.002294
CTGTCGC16650.034.1595469
TCTCGCT17700.032.565772
CTCGCTC17350.031.5615753
GTGTACG5150.031.0330451
GTCTTGC30400.030.8265611
GTATTAC6850.029.6945271
CGCTCTG18900.027.4268975
GTATTAG9200.026.8472651
GTATAGA9400.026.2760451
GTATTAT8700.025.8851931
GTCTAAC7650.025.63961
GTCTCAC38100.024.0245111
GTCTTAC12100.024.0150551
GTACGGA3051.9952495E-823.818211
GTGTAAC10100.023.735671
GGTATAC7450.023.4025881
GTGTATA15850.023.3749051
Produced by FastQC (version 0.11.5)