FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866393
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13250.044.4125751
TCTGTCG13450.039.5910958
TCGCTCT15850.036.773614
TCTCGCT17500.033.3063852
GTCTTGC27450.030.7009981
CTGTCGC17700.029.6782079
CTCGCTC18800.029.08953
CGCTCTG17800.027.894415
GTGTAAA17600.025.1799011
GTACTAG6400.024.9735091
CGTTTCG2051.3684497E-524.5715359
GTATTAT7750.024.3729721
GTATTAA9500.023.706911
TAATACT8400.023.1294354
GTATTAG9450.023.0635551
GTGTAAC9600.022.7031881
GTTTCAC41750.022.2338033
GTGTTAG19250.021.8894141
GTATAAG8300.021.007291
TTGCTCT41900.020.6085644
Produced by FastQC (version 0.11.5)