FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624299
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.033.176058
GTCTCGC11700.032.6218071
TCGCTCT11900.030.2517174
GTCTAGC8650.028.3061521
CTGTCGC12800.026.9996579
GTATTAG6950.025.904381
GTCTTGC20150.025.3747221
TCTCGCT13600.024.8872552
CGCTCTG13600.024.8820385
GTATTGG8200.024.5902061
GTATAAC5000.024.484821
CTCGCTC13450.021.9522783
TAACCCG2806.4733977E-620.5711695
TACACCG2806.4733977E-620.5711695
TTATACT6700.020.417654
GTATTAA7100.020.2856851
GTGTAAA13150.020.2625451
GTGTTAC9650.020.1490461
TACCCCG2908.767674E-619.8618165
GTCCTAA8750.019.752461
Produced by FastQC (version 0.11.5)