FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460191
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.043.4278538
CGGTTCG700.001093634241.1421745
CGTTTCG1401.5053956E-841.1421749
GTCTCGC10150.039.7323651
TCGCTCT11250.035.199424
CTGTCGC12450.031.8067049
TCTCGCT12050.031.6746082
GTCTTGC19450.030.7312981
CGCTCTG12150.029.6291395
GTACGGT1306.7452743E-427.6980443
CTCGCTC12250.027.630213
ATACCCG2405.6455974E-826.9995564
TACCCGT2207.96108E-726.1813875
GTACTAA4151.8189894E-1224.2941881
TTCCGCG1500.001554484824.004972
CTGTTGC22900.023.8948029
GTCTTAC6800.022.23991
GTTCTAC5550.022.058621
GTATTAG6950.021.7599011
GAGTGCA56300.021.4845496
Produced by FastQC (version 0.11.5)