FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2861727
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11000.039.926938
GTCTCGC11450.033.963161
CTGTCGC14000.031.3711599
TCGCTCT12200.030.6882614
GTCTTGC26500.030.4363081
TCTCGCT13250.028.2622872
CTCGCTC14150.025.9557483
GTGTACG4051.8189894E-1224.8938921
GTGTAGG16900.024.7150291
GTCTTAC11600.024.2117651
CGTTTCG1500.001556500323.9997929
GTGTAAG14000.023.66191
GTCTCAC32800.023.4925271
GTATTCG2151.9669495E-523.4465731
GTGTTAG18750.023.4287091
GTATTAG10500.023.3189741
GTGTAAC9300.023.2304761
ACCGTGT9000.023.19988
CGCTCTG15550.023.1509255
GTATTGC7500.023.0446321
Produced by FastQC (version 0.11.5)