FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2861727
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13150.039.9991461
TCTGTCG11950.039.7652058
AATACCG2507.6397555E-1131.6796135
GTCTTGC26300.031.2322081
CTGTCGC16800.030.8567669
TCGCTCT14450.030.3940934
TCTCGCT17250.029.6499612
TAATACT8350.029.3170074
CTCGCTC16300.029.1530193
CGCTCTG15700.028.8913655
GTATTAG10050.028.6779121
GGTATAC5700.027.8100281
GTGTTAC11450.027.6885851
GTGTAAT12350.025.0873661
AACCGTT1751.3170767E-424.685417
GTATAAG7600.024.6497961
GTCTCAC31750.023.3747561
GGATAAT12500.022.4806141
GTATTAA9950.022.4487531
TTGCTCT35150.022.1220634
Produced by FastQC (version 0.11.5)